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KMID : 0614019990150010295
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Journal of Pharmaceutical Sciences (C.N.U.)
1999 Volume.15 No. 1 p.295 ~ p.297
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Advanced Method for Determination of Omeprazole in Plasma by HPLC
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Kang Won-Ku
Kim Dong-Sup
Kwon Kwang-Il
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Abstract
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An advanced and sensitive high-performance liquid chromatographic (HPLC) method for determination of omeprazole in human plasma has been developed. After omeprazole was extracted from plasma with diethylether, the organic phase was transferred to another tube and trapped back with 0.1 N NAOH solution. The alkaline aqueous layer was injected into a reversed-phase C_8 column. Lansoprazole was used as an internal standard. The mobile phase consisted of 30% of acetonitrile and 70% of 0.2M KH_2PO_4, pH 7.0. Recoveries of the analytes and internal standard were >75.48%. The coefficients of variation of intra- and inter-day assay were <5.78 and 4.59% for plasma samples. The detection limit in plasma was 2ng/ml. The clinical applicability of this assay method was evaluated by determining plasma concentration-time courses of the respective analytes in 24 healthy volunteers after oral administration 40mg of omeprazole. The present assay is considered to be simple, accurate, economical and suitable for the study of the kinetic disposition of omeprazole in the body.
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KEYWORD
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Omeprazole, HPLC, Plasma, Human volunteers
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